Class information for:
Level 1: MEGAPRIMER//MULTIPLE SITE DIRECTED MUTAGENESIS//OVERLAP EXTENSION

Basic class information
Class id #P Avg. number of
references		 Database coverage
of references
15387 715 19.3 68%


Bar chart of Publication_year

Last years might be incomplete

Hierarchy of classes
The table includes all classes above and classes immediately below the current class.


Cluster id Level Cluster label #P
7 4 INFECTIOUS DISEASES//MICROBIOLOGY//VIROLOGY 1353914
33 3       JOURNAL OF BACTERIOLOGY//MICROBIOLOGY//MOLECULAR MICROBIOLOGY 107033
1609 2             RESTRICTION ENDONUCLEASE//BIOTECHNIQUES//RESTRICTION MODIFICATION SYSTEM 7100
15387 1                   MEGAPRIMER//MULTIPLE SITE DIRECTED MUTAGENESIS//OVERLAP EXTENSION 715

Terms with highest relevance score


rank Term termType Chi square Shr. of publ. in
class containing
term		 Class's shr. of
term's tot. occurrences		 #P with
term in
class
1 MEGAPRIMER authKW 209253 1% 70% 7
2 MULTIPLE SITE DIRECTED MUTAGENESIS authKW 170822 1% 100% 4
3 OVERLAP EXTENSION authKW 133451 1% 63% 5
4 BIOTECHNIQUES journal 119296 18% 2% 130
5 CARLOS CHAGAS FILHO authKW 85411 0% 100% 2
6 DPNI DIGESTION authKW 85411 0% 100% 2
7 5 ADAPTER authKW 42705 0% 100% 1
8 ALKALI DENATURED DNA authKW 42705 0% 100% 1
9 ALPHA CGTASE authKW 42705 0% 100% 1
10 ASYMMETRIC OVERLAP EXTENSION PCR authKW 42705 0% 100% 1

Web of Science journal categories


Rank Term Chi square Shr. of publ. in
class containing
term		 Class's shr. of
term's tot. occurrences		 #P with
term in
class
1 Biochemical Research Methods 8215 36% 0% 256
2 Biochemistry & Molecular Biology 6381 76% 0% 541
3 Genetics & Heredity 1106 17% 0% 124
4 Biotechnology & Applied Microbiology 369 11% 0% 78
5 Chemistry, Analytical 197 9% 0% 61
6 Cell Biology 32 6% 0% 40
7 Biology 13 2% 0% 14
8 Biophysics 12 3% 0% 19
9 Virology 6 1% 0% 9
10 History & Philosophy of Science 5 0% 0% 3

Address terms


Rank Term Chi square Shr. of publ. in
class containing
term		 Class's shr. of
term's tot. occurrences		 #P with
term in
class
1 BAISC MED 42705 0% 100% 1
2 BIOPATHOL DIAGOST IMAGING 42705 0% 100% 1
3 CARDIOL CHARLES BRUNEAU 42705 0% 100% 1
4 EXPLORATORY SCI LIFE SCI S 42705 0% 100% 1
5 LIFE SCI OCEAN BIOL 42705 0% 100% 1
6 MEDIATEURS PROT 42705 0% 100% 1
7 MOL ERS P ERVAT INT MDPI BEIJING OFF 42705 0% 100% 1
8 MRCIBLS 42705 0% 100% 1
9 NEURAL VIROL NEURAL ONCOL 42705 0% 100% 1
10 PROT PHOSPHORYLAT UNIT BIOCHEM MOL BIOL 42705 0% 100% 1

Journals


Rank Term Chi square Shr. of publ. in
class containing
term		 Class's shr. of
term's tot. occurrences		 #P with
term in
class
1 BIOTECHNIQUES 119296 18% 2% 130
2 GENE ANALYSIS TECHNIQUES 28162 1% 8% 8
3 DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY 16720 2% 3% 13
4 NUCLEIC ACIDS RESEARCH 15052 17% 0% 118
5 GENE 11085 10% 0% 71
6 ANALYTICAL BIOCHEMISTRY 8026 8% 0% 59
7 PCR-METHODS AND APPLICATIONS 6500 1% 3% 5
8 MOLECULAR BIOTECHNOLOGY 5887 2% 1% 16
9 MOLECULAR BIOLOGY 3986 3% 0% 21
10 BIOTECHNOLOGY EDUCATION 3283 0% 8% 1

Author Key Words


Rank Term Chi square Shr. of publ. in
class containing
term		 Class's shr. of
term's tot. occurrences		 #P with
term in
class		 LCSH search Wikipedia search
1 MEGAPRIMER 209253 1% 70% 7 Search MEGAPRIMER Search MEGAPRIMER
2 MULTIPLE SITE DIRECTED MUTAGENESIS 170822 1% 100% 4 Search MULTIPLE+SITE+DIRECTED+MUTAGENESIS Search MULTIPLE+SITE+DIRECTED+MUTAGENESIS
3 OVERLAP EXTENSION 133451 1% 63% 5 Search OVERLAP+EXTENSION Search OVERLAP+EXTENSION
4 CARLOS CHAGAS FILHO 85411 0% 100% 2 Search CARLOS+CHAGAS+FILHO Search CARLOS+CHAGAS+FILHO
5 DPNI DIGESTION 85411 0% 100% 2 Search DPNI+DIGESTION Search DPNI+DIGESTION
6 5 ADAPTER 42705 0% 100% 1 Search 5+ADAPTER Search 5+ADAPTER
7 ALKALI DENATURED DNA 42705 0% 100% 1 Search ALKALI+DENATURED+DNA Search ALKALI+DENATURED+DNA
8 ALPHA CGTASE 42705 0% 100% 1 Search ALPHA+CGTASE Search ALPHA+CGTASE
9 ASYMMETRIC OVERLAP EXTENSION PCR 42705 0% 100% 1 Search ASYMMETRIC+OVERLAP+EXTENSION+PCR Search ASYMMETRIC+OVERLAP+EXTENSION+PCR
10 ATAXIA 3 42705 0% 100% 1 Search ATAXIA+3 Search ATAXIA+3

Core articles
The table includes core articles in the class. The following variables is taken into account for the relevance score of an article in a cluster c:
(1) Number of references referring to publications in the class.
(2) Share of total number of active references referring to publications in the class.
(3) Age of the article. New articles get higher score than old articles.
(4) Citation rate, normalized to year.


Rank Reference # ref.
in cl.		 Shr. of ref. in
cl.		 Citations
1 LING, MM , ROBINSON, BH , (1997) APPROACHES TO DNA MUTAGENESIS: AN OVERVIEW.ANALYTICAL BIOCHEMISTRY. VOL. 254. ISSUE 2. P. 157 -178 71 47% 145
2 XU, JZ , ZHANG, WG , (2016) STRATEGIES USED FOR GENETICALLY MODIFYING BACTERIAL GENOME: SITE-DIRECTED MUTAGENESIS, GENE INACTIVATION, AND GENE OVER-EXPRESSION.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B. VOL. 17. ISSUE 2. P. 83 -99 35 38% 0
3 CHIU, J , MARCH, PE , LEE, R , TILLETT, D , (2004) SITE-DIRECTED, LIGASE-INDEPENDENT MUTAGENESIS (SLIM): A SINGLE-TUBE METHODOLOGY APPROACHING 100% EFFICIENCY IN 4 H.NUCLEIC ACIDS RESEARCH. VOL. 32. ISSUE 21. P. - 17 85% 145
4 XIE, ZH , SHI, XJ , (2009) FAST AND ALMOST 100% EFFICIENCY SITE-DIRECTED MUTAGENESIS BY THE MEGAPRIMER PCR METHOD.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS. VOL. 36. ISSUE 11. P. 1490 -1494 15 100% 9
5 WU, DG , GUO, XW , LU, J , SUN, X , LI, F , CHEN, YF , XIAO, DG , (2013) A RAPID AND EFFICIENT ONE-STEP SITE-DIRECTED DELETION, INSERTION, AND SUBSTITUTION MUTAGENESIS PROTOCOL.ANALYTICAL BIOCHEMISTRY. VOL. 434. ISSUE 2. P. 254-258 12 86% 4
6 ADACHI, Y , FUKUHARA, C , (2012) TA STRATEGY FOR RAPID AND EFFICIENT SITE-DIRECTED MUTAGENESIS.ANALYTICAL BIOCHEMISTRY. VOL. 431. ISSUE 1. P. 66 -68 13 81% 1
7 KIPRIYANOV, SM , PETRENKO, VA , (1991) DETERMINATION OF THE STRUCTURE-FUNCTIONAL ORGANIZATION OF VIRAL-PROTEINS BY LOCALIZED MUTAGENESIS - (REVIEW).MOLECULAR BIOLOGY. VOL. 25. ISSUE 1. P. 1-15 41 51% 0
8 LEFEVRE, F , MASSON, JM , (1996) DEVELOPMENTS IN SITE-DIRECTED MUTAGENESIS.BIOFUTUR. VOL. . ISSUE 154. P. A3-A14 21 81% 0
9 KUNKEL, TA , (1985) RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. VOL. 82. ISSUE 2. P. 488-492 9 60% 6302
10 WANG, C , WANG, TY , ZHANG, LY , GAO, XJ , WANG, XW , JIN, CJ , (2015) CUT-AND-PASTE-BASED CLONING STRATEGY FOR LARGE GENE SITE-DIRECTED MUTAGENESIS.GENETICS AND MOLECULAR RESEARCH. VOL. 14. ISSUE 2. P. 5585 -5591 13 72% 0

Classes with closest relation at Level 1


Rank Class id link
1 12753 T VECTOR//TA CLONING//GENE SYNTHESIS
2 31018 BACTERIOPHAGE T5//22 KD ZEIN MESSENGER RNAS//CHEMICOENZYMATIC SYNTHESIS
3 10945 DIRECTED EVOLUTION//DNA SHUFFLING//RANDOM MUTAGENESIS
4 33383 SPEL 6//AND PLASMIDS//ASSEMBLY OF DNA FRAGMENTS
5 15724 FILAMENTOUS BACTERIOPHAGE//GENE 3 PROTEIN//GENE 5 PROTEIN
6 21589 COMPUTER APPLICATIONS IN THE BIOSCIENCES//ADAPTIVE WEIGHTED LEAST SQUARES//AMBIGRAM
7 25273 CMLA//CAPTURE ELEMENT//CATB7
8 784 RESTRICTION ENDONUCLEASE//RESTRICTION MODIFICATION SYSTEM//RESTRICTION MODIFICATION
9 30766 4D5SCFV//CSPBAT//BARNASE BARSTAR MODULE
10 20867 ADSORBING PROCESS//ATP UTILIZING ENZYMES//LC ILCJP

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