Carbohydrate structure and chemistry
Chemical, enzymatic, chromatographic, electrophoretic, and spectroscopic, especially mass spectrometric methods in polysaccharide analysis
Determination of the substitution pattern of polysaccharide derivatives with focus on cellulose ethers
Eight lectures including small exercises, home assignment/presentation
Give reasons for the importance of structure elucidation of polysaccharides
Activate knowledge on carbohydrate structure and chemistry with respect to error sources in analysis
Explain the general approach of comprehensive polysaccharide analysis
Describe the principle and methods for
- the sugar constituent analysis of polysaccharides
- the linkage pattern analysis
- oligosaccharide sequencing by mass spectrometry
- molecular mass determination
- location of non-sugar substituents in polysaccharide derivatives
Give examples for the application of enzymes in polysaccharide analysis
Know methods of quantification in carbohydrate