Project 3A The calcium-dependent antibody binding allows for purification with mild elution conditions Elution of captured antibodies with EDTA gives an equally sharp elution peak as acidic elution Sodium chloride can also be used to achieve a calcium-dependent elution in the absence of calcium Different subclasses require different pH for NaCl-mediated elution due to different affinities No aggregates detected in the eluate for an IgG4 compared to 34% aggregates in MabSelect SuRe eluate Previous Play/pause Next
