A new strategy to add substrate in perfusion process has been developed --> Targeted feeding (TAFE) Successful application of TAFE to define the addition of different sugars in CHO cell perfusion process --> control of sugar/ lactate metabolism --> cell specific consumption rates of the different sugars are according to the targets decided a priori by the operator --> it is now possible to feed non-glucose sugars in presence of glucose
Successful application of TAFE to define the addition of different sugars in CHO cell perfusion process various targets of cell specific sugar consumption rates generate different glycosylation profiles tool for glycosylation tuning
Our mathematical model (GReBA) can be used to decide the targets of cell specific sugar consumption rates to be used in TAFE feeding
