Cell culture laboratory: equipment, appliances, consumables. Cell culture methods. Media and solutions. Contamination of cell cultures. Cell viability tests. Good cell culture practice. Ethical and sustainability issues related to the cell culture.
SK2513 Cell Culture: Theory and Practice 4.0 credits
Information per course offering
Course offerings are missing for current or upcoming semesters.
Course syllabus as PDF
Please note: all information from the Course syllabus is available on this page in an accessible format.
Course syllabus SK2513 (Autumn 2020–)Headings with content from the Course syllabus SK2513 (Autumn 2020–) are denoted with an asterisk ( )
Content and learning outcomes
Course contents
Intended learning outcomes
After completing the course the student should be able to:
- independently subculture an animal cell line
- describe the equipment, appliances, and consumables needed for cell culture
- account for the different aspects of good cell culture practice
- perform calculations necessary for a cell line propagation and for preparation of stock solutions
Literature and preparations
Specific prerequisites
For single courses students, 120 hp is required in engineering and natural sciences or equivalent, as well as knowledge of English B or equivalent.
Recommended prerequisites
SK2530 (Introduction to Biomedicine) or SK2531 (Biomedicine for Engineers) is recommended, but is not a requirement.
Equipment
No information inserted
Literature
No information inserted
Examination and completion
If the course is discontinued, students may request to be examined during the following two academic years.
Grading scale
A, B, C, D, E, FX, F
Examination
- LAB1 - Laboratory experiments, 2.0 credits, grading scale: P, F
- TEN1 - Written exam, 2.0 credits, grading scale: A, B, C, D, E, FX, F
Based on recommendation from KTH’s coordinator for disabilities, the examiner will decide how to adapt an examination for students with documented disability.
The examiner may apply another examination format when re-examining individual students.
Other requirements for final grade
Laboratory exercises (LAB1 2,0 credits, grade: P)
Written exam (TEN1 2,0 credits, grade scale: A, B, C, D, E)
Opportunity to complete the requirements via supplementary examination
No information inserted
Opportunity to raise an approved grade via renewed examination
Yes
Examiner
Ethical approach
- All members of a group are responsible for the group's work.
- In any assessment, every student shall honestly disclose any help received and sources used.
- In an oral assessment, every student shall be able to present and answer questions about the entire assignment and solution.
Further information
Course room in Canvas
Registered students find further information about the implementation of the course in the course room in Canvas. A link to the course room can be found under the tab Studies in the Personal menu at the start of the course.
Offered by
Main field of study
Biotechnology
Education cycle
Second cycle
Add-on studies
No information inserted
Contact
Marina Zelenina (marinaz@kth.se)