Publications NYGREN, P-Å

- Publication list Per-Åke Nygren -

Ozawa T, Morikawa M, Morishita Y, Ogikubo K, Itoh F, Koinuma D, Nygren P-Å, Miyazono K. Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice. iScience. 2021 May 14;24(5):102488. doi: 10.1016/j.isci.2021.102488. PMID: 34113826; PMCID: PMC8170004.

Yu W, Gillespie KP, Chhay B, Svensson AS, Nygren P-Å, Blair IA, Yu F, Tsourkas A. Efficient Labeling of Native Human IgG by Proximity-Based Sortase-Mediated Isopeptide Ligation. Bioconjug Chem. 2021 Jun 16;32(6):1058-1066. doi: 10.1021/acs.bioconjchem.1c00099. Epub 2021 May 24. PMID: 34029057.

Duru AD, Sun R, Allerbring EB, Chadderton J, Kadri N, Han X, Peqini K, Uchtenhagen H, Madhurantakam C, Pellegrino S, Sandalova T, Nygren P-Å, Turner SJ, Achour A. Tuning antiviral CD8 T-cell response via proline-altered peptide ligand vaccination. PLoS Pathog. 2020 May 4;16(5):e1008244. doi: 10.1371/journal.ppat.1008244. PMID: 32365082; PMCID: PMC7224568.

Schulte, T., Sala, B., Nilvebrant, J., Nygren, P-Å., Achour, A., Shernyukov, A., Agback, T., Agback, P. (2020), Assigned NMR backbone resonances of the ligand-binding region domain of the pneumococcal Serine-Rich Repeat Protein (PsrP-BR) reveal a rigid monomer in solution, Biomol. NMR ass., doi.org/10.1007/s12104-020-09944

Hjelm LC, Nilvebrant J, Nygren PÅ, Nilsson AS, Seijsing J. Lysis of Staphylococcal Cells by Modular Lysin Domains Linked via a Non-covalent Barnase-Barstar Interaction Bridge. Front Microbiol. 2019 Mar 22;10:558. doi:10.3389/fmicb.2019.00558. eCollection 2019. PubMed PMID: 30967850; PubMed Central PMCID: PMC6439198.

Hafstrand I, Sayitoglu EC, Apavaloaei A, Josey BJ, Sun R, Han X, Pellegrino S, Ozkazanc D, Potens R, Janssen L, Nilvebrant J, Nygren PÅ, Sandalova T, Springer S, Georgoudaki AM, Duru AD, Achour A. Successive crystal structure snapshots suggest the basis for MHC class I peptide loading and editing by tapasin. Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):5055-5060. doi: 10.1073/pnas.1807656116. Epub 2019 Feb 26. PubMed PMID: 30808808; PubMed Central PMCID: PMC6421438.

Lundqvist M, Thalén N, Volk AL, Hansen HG, von Otter E, Nygren PÅ, Uhlen M, Rockberg J. Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion. Sci Rep. 2019 Jan 22;9(1):310. doi: 10.1038/s41598-018-36559-x. PubMed PMID: 30670736; PubMed Central PMCID: PMC6342966.

Subramanian K, Neill DR, Malak HA, Spelmink L, Khandaker S, Dalla Libera Marchiori G, Dearing E, Kirby A, Yang M, Achour A, Nilvebrant J, Nygren PÅ, Plant L, Kadioglu A, Henriques-Normark B. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol. 2019 Jan;4(1):62-70. doi: 10.1038/s41564-018-0280-x. Epub 2018 Nov 12. PubMed PMID: 30420782; PubMed Central PMCID: PMC6298590.

Yu F, Alesand V, Nygren PÅ. Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation. Biotechnol J. 2018 Jul;13(7):e1700688. doi: 10.1002/biot.201700688. Epub 2018 Mar 14. PubMed PMID: 29485240.

Ståhl S, Gräslund T, Eriksson Karlström A, Frejd FY, Nygren PÅ, Löfblom J. Affibody Molecules in Biotechnological and Medical Applications. Trends Biotechnol. 2017 Aug;35(8):691-712. doi: 10.1016/j.tibtech.2017.04.007. Epub 2017 May 14. Review. PubMed PMID: 28514998.

Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A. The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep. 2016 Sep 1;6:32371. doi: 10.1038/srep32371. PubMed PMID: 27582320; PubMed Central PMCID: PMC5007671.

Shibasaki S, Karasaki M, Gräslund T, Nygren PÅ, Sano H, Iwasaki T. Inhibitory  effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function. AMB  Express. 2014 Dec;4(1):82. doi: 10.1186/s13568-014-0082-3. Epub 2014 Nov 11. PubMed PMID: 26267111; PubMed Central PMCID: PMC4884024.

Yu F, Gudmundsdotter L, Akal A, Gunneriusson E, Frejd F, Nygren PÅ. An affibody-adalimumab hybrid blocks combined IL-6 and TNF-triggered serum amyloid A secretion in vivo. MAbs. 2014;6(6):1598-607. doi: 10.4161/mabs.36089. PubMed PMID: 25484067; PubMed Central PMCID: PMC4622551.

Jansson R, Thatikonda N, Lindberg D, Rising A, Johansson J, Nygren PÅ, Hedhammar M. Recombinant spider silk genetically functionalized with affinity domains. Biomacromolecules. 2014 May 12;15(5):1696-706. doi: 10.1021/bm500114e. Epub 2014 Apr 8. PubMed PMID: 24678858.

Neiman M, Fredolini C, Johansson H, Lehtiö J, Nygren PÅ, Uhlén M, Nilsson P,  Schwenk JM. Selectivity analysis of single binder assays used in plasma protein profiling. Proteomics. 2013 Dec;13(23-24):3406-10. doi: 10.1002/pmic.201300030. Epub 2013 Nov 18. PubMed PMID: 24151238; PubMed Central PMCID: PMC4265267.

Uchtenhagen H, Abualrous ET, Stahl E, Allerbring EB, Sluijter M, Zacharias M, Sandalova T, van Hall T, Springer S, Nygren PÅ, Achour A. Proline substitution independently enhances H-2D(b) complex stabilization and TCR recognition of melanoma-associated peptides. Eur J Immunol. 2013 Nov;43(11):3051-60. doi: 10.1002/eji.201343456. Epub 2013 Aug 30. PubMed PMID: 23939911.

Yu F, Järver P, Nygren PÅ. Tailor-making a protein a-derived domain for efficient site-specific photocoupling to Fc of mouse IgG₁. PLoS One. 2013;8(2):e56597. doi: 10.1371/journal.pone.0056597. Epub 2013 Feb 12. PubMed PMID: 23424669; PubMed Central PMCID: PMC3570467.

Allerbring EB, Duru AD, Uchtenhagen H, Madhurantakam C, Tomek MB, Grimm S, Mazumdar PA, Friemann R, Uhlin M, Sandalova T, Nygren PÅ, Achour A. Unexpected T-cell recognition of an altered peptide ligand is driven by reversed thermodynamics. Eur J Immunol. 2012 Nov;42(11):2990-3000. doi: 10.1002/eji.201242588. Epub 2012 Sep 20. PubMed PMID: 22837158.

Akter F, Mie M, Grimm S, Nygren PÅ, Kobatake E. Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*. Anal Chem. 2012 Jun 5;84(11):5040-6. doi: 10.1021/ac300708r. Epub 2012 May 16. PubMed PMID: 22571843.

Grimm S, Salahshour S, Nygren PÅ. Monitored whole gene in vitro evolution of  an anti-hRaf-1 affibody molecule towards increased binding affinity. N Biotechnol. 2012 Jun 15;29(5):534-42. doi: 10.1016/j.nbt.2011.10.008. Epub 2011 Oct 19. PubMed PMID: 22027369.

Nygren PÅ. Affinity ligands from biological combinatorial libraries. Methods  Biochem Anal. 2011;54:269-78. Review. PubMed PMID: 21954782.

Grimm S, Yu F, Nygren PÅ. Ribosome display selection of a murine IgG₁ Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies. Mol Biotechnol. 2011 Jul;48(3):263-76. doi: 10.1007/s12033-010-9367-1. PubMed PMID: 21197589; PubMed Central PMCID: PMC3115053.

Grimm S, Lundberg E, Yu F, Shibasaki S, Vernet E, Skogs M, Nygren PÅ, Gräslund T. Selection and characterisation of affibody molecules inhibiting the interaction between Ras and Raf in vitro. N Biotechnol. 2010 Dec 31;27(6):766-73. doi: 10.1016/j.nbt.2010.07.016. Epub 2010 Jul 30. PubMed PMID: 20674812.

Bourbeillon J, Orchard S, Benhar I, Borrebaeck C, de Daruvar A, Dübel S, Frank R, Gibson F, Gloriam D, Haslam N, Hiltker T, Humphrey-Smith I, Hust M, Juncker D, Koegl M, Konthur Z, Korn B, Krobitsch S, Muyldermans S, Nygren PÅ, Palcy S, Polic B, Rodriguez H, Sawyer A, Schlapshy M, Snyder M, Stoevesandt O, Taussig MJ, Templin M, Uhlen M, van der Maarel S, Wingren C, Hermjakob H, Sherman D. Minimum information about a protein affinity reagent (MIAPAR). Nat Biotechnol. 2010 Jul;28(7):650-3. doi: 10.1038/nbt0710-650. PubMed PMID: 20622827.

Löfdahl PA, Nygren PÅ. Affinity maturation of a TNFalpha-binding affibody molecule by Darwinian survival selection. Biotechnol Appl Biochem. 2010 Mar 5;55(3):111-20. doi: 10.1042/BA20090274. PubMed PMID: 20121700.

Löfdahl PA, Nord O, Janzon L, Nygren PÅ. Selection of TNF-alpha binding affibody molecules using a beta-lactamase protein fragment complementation assay. N Biotechnol. 2009 Nov 30;26(5):251-9. doi: 10.1016/j.nbt.2009.06.980. Epub 2009  Jul 1. PubMed PMID: 19576305.

Vernet E, Lundberg E, Friedman M, Rigamonti N, Klausing S, Nygren PÅ, Gräslund T. Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain. N Biotechnol. 2009 Sep;25(6):417-23. doi: 10.1016/j.nbt.2009.02.001. PubMed PMID: 19552886.

Vernet E, Konrad A, Lundberg E, Nygren PÅ, Gräslund T. Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule. J Immunol Methods. 2008 Sep 30;338(1-2):1-6. doi: 10.1016/j.jim.2008.06.005. Epub 2008 Jul 29. PubMed PMID: 18671978.

Jonsson A, Dogan J, Herne N, Abrahmsén L, Nygren PÅ. Engineering of a femtomolar affinity binding protein to human serum albumin. Protein Eng Des Sel.  2008 Aug;21(8):515-27. doi: 10.1093/protein/gzn028. Epub 2008 May 22. PubMed PMID: 18499681.

Nygren PÅ. Alternative binding proteins: affibody binding proteins developed  from a small three-helix bundle scaffold. FEBS J. 2008 Jun;275(11):2668-76. doi:  10.1111/j.1742-4658.2008.06438.x. Epub 2008 Apr 24. Review. PubMed PMID: 18435759.

Wagner S, Baars L, Ytterberg AJ, Klussmeier A, Wagner CS, Nord O, Nygren PÅ,  van Wijk KJ, de Gier JW. Consequences of membrane protein overexpression in Escherichia coli. Mol Cell Proteomics. 2007 Sep;6(9):1527-50. Epub 2007 Apr 19. PubMed PMID: 17446557.

Bandmann N, Nygren PÅ. Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res. 2007;35(5):e32. Epub 2007 Jan 30. PubMed PMID: 17264122; PubMed Central PMCID:PMC1865067.

Renberg B, Nordin J, Merca A, Uhlén M, Feldwisch J, Nygren PÅ, Karlström AE. Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats. J Proteome Res. 2007 Jan;6(1):171-9.PubMed PMID: 17203961.

Taussig MJ, Stoevesandt O, Borrebaeck CA, Bradbury AR, Cahill D, Cambillau C, de Daruvar A, Dübel S, Eichler J, Frank R, Gibson TJ, Gloriam D, Gold L, Herberg  FW, Hermjakob H, Hoheisel JD, Joos TO, Kallioniemi O, Koegl M, Konthur Z, Korn B, Kremmer E, Krobitsch S, Landegren U, van der Maarel S, McCafferty J, Muyldermans  S, Nygren PÅ, Palcy S, Plückthun A, Polic B, Przybylski M, Saviranta P, Sawyer A, Sherman DJ, Skerra A, Templin M, Ueffing M, Uhlén M. ProteomeBinders: planning a  European resource of affinity reagents for analysis of the human proteome. Nat Methods. 2007 Jan;4(1):13-7. Erratum in: Nat Methods. 2007 Feb;4(2):187. Koegll,  Manfred [corrected to Koegl, Manfred]. PubMed PMID: 17195019.

Myhre S, Henning P, Granio O, Tylö AS, Nygren PÅ, Olofsson S, Boulanger P, Lindholm L, Hong SS. Decreased immune reactivity towards a knobless, affibody-targeted adenovirus type 5 vector. Gene Ther. 2007 Feb;14(4):376-81. Epub 2006 Oct 12. PubMed PMID: 17036056.

Engfeldt T, Renberg B, Brumer H, Nygren PÅ, Karlström AE. Chemical synthesis  of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein. Chembiochem. 2005 Jun;6(6):1043-50. PubMed PMID: 15880677.

Wiklund M, Nord O, Gothäll R, Chernyshev AV, Nygren PÅ, Hertz HM. Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay. Anal Biochem. 2005  Mar 1;338(1):90-101. PubMed PMID: 15707939.

Nord O, Gustrin A, Nygren PÅ. Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation. FEMS Microbiol Lett. 2005 Jan 1;242(1):73-9. PubMed PMID: 15621422.

Henning P, Andersson KM, Frykholm K, Ali A, Magnusson MK, Nygren PÅ, Granio O, Hong SS, Boulanger P, Lindholm L. Tumor cell targeted gene delivery by adenovirus 5 vectors carrying knobless fibers with antibody-binding domains. Gene Ther. 2005 Feb;12(3):211-24. PubMed PMID: 15510176.

Nygren PÅ, Skerra A. Binding proteins from alternative scaffolds. J Immunol Methods. 2004 Jul;290(1-2):3-28. Review. PubMed PMID: 15261569.

Lendel C, Dincbas-Renqvist V, Flores A, Wahlberg E, Dogan J, Nygren PÅ, Härd  T. Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A. Protein Sci. 2004 Aug;13(8):2078-88. Epub 2004 Jul 6. PubMed PMID: 15238637; PubMed Central PMCID: PMC2279809.

Eklund M, Sandström K, Teeri TT, Nygren PÅ. Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. J Biotechnol. 2004 Apr 29;109(3):277-86. PubMed PMID: 15066765.

Linhult M, Gülich S, Gräslund T, Nygren PÅ, Hober S. Evaluation of different  linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand. Protein Eng. 2003 Dec;16(12):1147-52. PubMed PMID: 14983098.

Andersson M, Rönnmark J, Areström I, Nygren PÅ, Ahlborg N. Inclusion of a non-immunoglobulin binding protein in two-site ELISA for quantification of human  serum proteins without interference by heterophilic serum antibodies. J Immunol Methods. 2003 Dec;283(1-2):225-34. PubMed PMID: 14659914.

Nord O, Uhlén M, Nygren PÅ. Microbead display of proteins by cell-free expression of anchored DNA. J Biotechnol. 2003 Dec 5;106(1):1-13. PubMed PMID:14636705.

Rönnmark J, Kampf C, Asplund A, Höidén-Guthenberg I, Wester K, Pontén F, Uhlén M, Nygren PÅ. Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol. J Immunol Methods. 2003 Oct 1;281(1-2):149-60. PubMed PMID: 14580889.

Sandström K, Xu Z, Forsberg G, Nygren PÅ. Inhibition of the CD28-CD80  co-stimulation signal by a CD28-binding affibody ligand developed by combinatorial protein engineering. Protein Eng. 2003 Sep;16(9):691-7. PubMed PMID: 14560055.

Högbom M, Eklund M, Nygren PÅ, Nordlund P. Structural basis for recognition by an in vitro evolved affibody. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3191-6. Epub 2003 Feb 25. PubMed PMID: 12604795; PubMed Central PMCID:PMC404300.

Wahlberg E, Lendel C, Helgstrand M, Allard P, Dincbas-Renqvist V, Hedqvist A, Berglund H, Nygren PÅ, Härd T. An affibody in complex with a target protein: structure and coupled folding. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3185-90. Epub 2003 Feb 19. PubMed PMID: 12594333; PubMed Central PMCID: PMC152267.

Lendel C, Wahlberg E, Berglund H, Eklund M, Nygren PÅ, Härd T. 1H, 13C and 15N resonance assignments of an affibody-target complex. J Biomol NMR. 2002 Nov;24(3):271-2. PubMed PMID: 12522315.

Henning P, Magnusson MK, Gunneriusson E, Hong SS, Boulanger P, Nygren PÅ, Lindholm L. Genetic modification of adenovirus 5 tropism by a novel class of ligands based on a three-helix bundle scaffold derived from staphylococcalprotein A. Hum Gene Ther. 2002 Aug 10;13(12):1427-39. PubMed PMID: 12215264.

Gräslund S, Eklund M, Falk R, Uhlén M, Nygren PÅ, Ståhl S. A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products. J Biotechnol. 2002 Oct 9;99(1):41-50. PubMed PMID: 12204556.

Gräslund T, Hedhammar M, Uhlén M, Nygren PÅ, Hober S. Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology. J Biotechnol. 2002 Jun 13;96(1):93-102. PubMed PMID: 12142146.

Eklund M, Axelsson L, Uhlén M, Nygren PÅ. Anti-idiotypic protein domains selected from protein A-based affibody libraries. Proteins. 2002 Aug 15;48(3):454-62. PubMed PMID: 12112671.

Rönnmark J, Grönlund H, Uhlén M, Nygren PÅ. Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A. Eur J Biochem. 2002 Jun;269(11):2647-55. PubMed PMID: 12047372.

Samuelson P, Gunneriusson E, Nygren PÅ, Ståhl S. Display of proteins on bacteria. J Biotechnol. 2002 Jun 26;96(2):129-54. Review. PubMed PMID: 12039531.

Jonasson P, Liljeqvist S, Nygren PÅ, Ståhl S. Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli. Biotechnol Appl Biochem. 2002 Apr;35(Pt 2):91-105. Review. PubMed PMID: 11916451.

Rönnmark J, Hansson M, Nguyen T, Uhlén M, Robert A, Ståhl S, Nygren PÅ. Construction and characterization of affibody-Fc chimeras produced in Escherichia coli. J Immunol Methods. 2002 Mar 1;261(1-2):199-211. PubMed PMID: 11861078.

Gräslund T, Ehn M, Lundin G, Hedhammar M, Uhlén M, Nygren PÅ, Hober S. Strategy for highly selective ion-exchange capture using a charge-polarizing fusion partner. J Chromatogr A. 2002 Jan 4;942(1-2):157-66. PubMed PMID: 11822381.

Johansson MU, Frick IM, Nilsson H, Kraulis PJ, Hober S, Jonasson P, Linhult M, Nygren PÅ, Uhlén M, Björck L, Drakenberg T, Forsén S, Wikström M. Structure, specificity, and mode of interaction for bacterial albumin-binding modules. J Biol Chem. 2002 Mar 8;277(10):8114-20. Epub 2001 Dec 18. PubMed PMID: 11751858.

Bandmann N, Van Alstine J, Veide A, Nygren PÅ. Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins. J Biotechnol. 2002 Jan 31;93(1):1-14. PubMed PMID: 11690690.

Wernérus H, Lehtiö J, Teeri T, Nygren PÅ, Ståhl S. Generation of metal-binding staphylococci through surface display of combinatorially engineered cellulose-binding domains. Appl Environ Microbiol. 2001 Oct;67(10):4678-84. PubMed PMID: 11571172; PubMed Central PMCID: PMC93219.

Nord K, Nord O, Uhlén M, Kelley B, Ljungqvist C, Nygren PÅ. Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A. Eur J Biochem. 2001 Aug;268(15):4269-77. PubMed PMID: 11488921.

Karlström A, Nygren PÅ. Dual labeling of a binding protein allows for specific fluorescence detection of native protein. Anal Biochem. 2001 Aug 1;295(1):22-30. PubMed PMID: 11476541.

Danielsen S, Eklund M, Deussen HJ, Gräslund T, Nygren PÅ, Borchert TV. In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor. Gene. 2001 Jul 11;272(1-2):267-74. PubMed PMID: 11470533.

Gräslund T, Lundin G, Uhlén M, Nygren PÅ, Hober S. Charge engineering of a protein domain to allow efficient ion-exchange recovery. Protein Eng. 2000 Oct;13(10):703-9. PubMed PMID: 11112509.

Lehtiö J, Teeri TT, Nygren PÅ. Alpha-amylase inhibitors selected from a combinatorial library of a cellulose binding domain scaffold. Proteins. 2000 Nov 15;41(3):316-22. PubMed PMID: 11025543.

Hansson M, Nygren PÅ, Ståhl S. Design and production of recombinant subunit vaccines. Biotechnol Appl Biochem. 2000 Oct;32 ( Pt 2):95-107. Review. PubMed PMID: 11001870.

Berggren K, Nilsson A, Johansson G, Bandmann N, Nygren PÅ, Tjerneld F. Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure. J Chromatogr B Biomed Sci Appl. 2000 Jun 23;743(1-2):295-306. PubMed PMID:10942300.

Nord K, Gunneriusson E, Uhlén M, Nygren PÅ. Ligands selected from combinatorial libraries of protein A for use in affinity capture of apolipoprotein A-1M and taq DNA polymerase. J Biotechnol. 2000 Jun 9;80(1):45-54. PubMed PMID: 10862985.

Bandmann N, Collet E, Leijen J, Uhlén M, Veide A, Nygren PÅ. Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems. J Biotechnol. 2000 Apr 28;79(2):161-72. PubMed PMID: 10812184.

Legendre D, Laraki N, Gräslund T, Bjørnvad ME, Bouchet M, Nygren PÅ, Borchert TV, Fastrez J. Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors. J Mol Biol. 2000 Feb 11;296(1):87-102. PubMed PMID: 10656819.

Jonasson P, Nygren PÅ, Jörnvall H, Johansson BL, Wahren J, Uhlén M, Ståhl S. Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein. J Biotechnol. 2000 Jan 21;76(2-3):215-26. PubMed PMID: 10656336.

Andersson K, Gülich S, Hämäläinen M, Nygren PÅ, Hober S, Malmqvist M. Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space. Proteins. 1999 Nov 15;37(3):494-8. PubMed PMID: 10591108.

Gunneriusson E, Samuelson P, Ringdahl J, Grönlund H, Nygren PÅ, Ståhl S. Staphylococcal surface display of immunoglobulin A (IgA)- and IgE-specific in vitro-selected binding proteins (affibodies) based on Staphylococcus aureus protein A. Appl Environ Microbiol. 1999 Sep;65(9):4134-40. PubMed PMID: 10473426;PubMed Central PMCID: PMC99751.

Hansson M, Ringdahl J, Robert A, Power U, Goetsch L, Nguyen TN, Uhlén M, Ståhl S, Nygren PÅ. An in vitro selected binding protein (affibody) shows conformation-dependent recognition of the respiratory syncytial virus (RSV) G protein. Immunotechnology. 1999 Mar;4(3-4):237-52. PubMed PMID: 10231093.

Berggren K, Veide A, Nygren PÅ, Tjerneld F. Genetic engineering of protein-peptide fusions for control of protein partitioning in thermoseparating aqueous two-phase systems. Biotechnol Bioeng. 1999 Jan 20;62(2):135-44. PubMedPMID: 10099522.

Nilsson P, Larsson A, Lundeberg J, Uhlén M, Nygren PÅ. Mutational scanning of PCR products by subtractive oligonucleotide hybridization analysis. Biotechniques. 1999 Feb;26(2):308-16. PubMed PMID: 10023543.

Jonasson P, Nygren PÅ, Johansson BL, Wahren J, Uhlén M, Ståhl S. Gene fragment polymerization gives increased yields of recombinant human proinsulin C-peptide. Gene. 1998 Apr 14;210(2):203-10. PubMed PMID: 9573365.

Jansson B, Uhlén M, Nygren PÅ. All individual domains of staphylococcal protein A show Fab binding. FEMS Immunol Med Microbiol. 1998 Jan;20(1):69-78. PubMed PMID: 9514577.

O'Meara D, Nilsson P, Nygren PÅ, Uhlén M, Lundeberg J. Capture of single-stranded DNA assisted by oligonucleotide modules. Anal Biochem. 1998 Jan 15;255(2):195-203. PubMed PMID: 9451504.

Nilsson J, Ståhl S, Lundeberg J, Uhlén M, Nygren PÅ. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif. 1997 Oct;11(1):1-16. Review. PubMed PMID: 9325133.

Nygren PÅ, Uhlén M. Scaffolds for engineering novel binding sites in proteins. Curr Opin Struct Biol. 1997 Aug;7(4):463-9. Review. PubMed PMID: 9266166.

Nord K, Gunneriusson E, Ringdahl J, Ståhl S, Uhlén M, Nygren PÅ. Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat Biotechnol. 1997 Aug;15(8):772-7. PubMed PMID: 9255793.

Ahlborg N, Sterky F, Haddad D, Perlmann P, Nygren PÅ, Andersson R, Berzins K. Predominance of H-2d- and H-2k-restricted T-cell epitopes in the highly repetitive Plasmodium falciparum antigen Pf332. Mol Immunol. 1997 Apr;34(5):379-89. PubMed PMID: 9293771.

Nilsson J, Bosnes M, Larsen F, Nygren PÅ, Uhlén M, Lundeberg J. Heat-mediated activation of affinity-immobilized Taq DNA polymerase. Biotechniques. 1997 Apr;22(4):744-51. PubMed PMID: 9105627.

Sjölander A, Nygren PÅ, Stahl S, Berzins K, Uhlen M, Perlmann P, Andersson R. The serum albumin-binding region of streptococcal protein G: a bacterial fusion partner with carrier-related properties. J Immunol Methods. 1997 Feb 14;201(1):115-23. PubMed PMID: 9032414.

Jendeberg L, Nilsson P, Larsson A, Denker P, Uhlén M, Nilsson B Nygren PÅ. Engineering of Fc(1) and Fc(3) from human immunoglobulin G to analyse subclass specificity for staphylococcal protein A. J Immunol Methods. 1997 Feb14;201(1):25-34. PubMed PMID: 9032407.

Gräslund T, Nilsson J, Lindberg AM, Uhlén M, Nygren PÅ. Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein. Protein Expr Purif. 1997 Feb;9(1):125-32. PubMed PMID: 9116494.

Nilsson P, Persson B, Larsson A, Uhlén M, Nygren PÅ. Detection of mutations in PCR products from clinical samples by surface plasmon resonance. J Mol Recognit. 1997 Jan-Feb;10(1):7-17. PubMed PMID: 9179775.

Ståhl S, Nygren PÅ, Uhlén M. Detection and isolation of recombinant proteins based on binding affinity of reporter: protein A. Methods Mol Biol. 1997;63:103-18. PubMed PMID: 9113645.

Ståhl S, Nygren PÅ, Uhlén M. Strategies for gene fusions. Methods Mol Biol. 1997;62:37-54. PubMed PMID: 9108511.

Ståhl S, Nygren PÅ. The use of gene fusions to protein A and protein G in immunology and biotechnology. Pathol Biol (Paris). 1997 Jan;45(1):66-76. Review. PubMed PMID: 9097850.

Nilsson J, Larsson M, Ståhl S, Nygren PÅ, Uhlén M. Multiple affinity domains for the detection, purification and immobilization of recombinant proteins. J Mol Recognit. 1996 Sep-Dec;9(5-6):585-94. PubMed PMID: 9174944.

Makrides SC, Nygren PÅ, Andrews B, Ford PJ, Evans KS, Hayman EG, Adari H, Uhlén M, Toth CA. Extended in vivo half-life of human soluble complement receptor type 1 fused to a serum albumin-binding receptor. J Pharmacol Exp Ther. 1996 Apr;277(1):534-42. PubMed PMID: 8613964.

Gunneriusson E, Samuelson P, Uhlen M, Nygren PÅ, Stähl S. Surface display of a functional single-chain Fv antibody on staphylococci. J Bacteriol. 1996 Mar;178(5):1341-6. PubMed PMID: 8631711; PubMed Central PMCID: PMC177808.

Kraulis PJ, Jonasson P, Nygren PÅ, Uhlén M, Jendeberg L, Nilsson B, Kördel J. The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study. FEBS Lett. 1996 Jan 8;378(2):190-4. PubMed PMID: 8549831.

Nguyen TN, Gourdon MH, Hansson M, Robert A, Samuelson P, Libon C, Andréoni C, Nygren PÅ, Binz H, Uhlén M, et al. Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus. J Biotechnol. 1995 Oct 16;42(3):207-19. PubMed PMID: 7576540.

Karlsson R, Jendeberg L, Nilsson B, Nilsson J, Nygren PÅ. Direct and competitive kinetic analysis of the interaction between human IgG1 and a one domain analogue of protein A. J Immunol Methods. 1995 Jun 14;183(1):43-9. PubMedPMID: 7602138.

Nord K, Nilsson J, Nilsson B, Uhlén M, Nygren PÅ. A combinatorial library of an alpha-helical bacterial receptor domain. Protein Eng. 1995 Jun;8(6):601-8. PubMed PMID: 8532685.

Nilsson P, Persson B, Uhlén M, Nygren PÅ. Real-time monitoring of DNA manipulations using biosensor technology. Anal Biochem. 1995 Jan 1;224(1):400-8. PubMed PMID: 7710099.

Nilsson J, Nilsson P, Williams Y, Pettersson L, Uhlén M, Nygren PÅ. Competitive elution of protein A fusion proteins allows specific recovery under mild conditions. Eur J Biochem. 1994 Aug 15;224(1):103-8. PubMed PMID: 8076629.

Nygren PÅ, Ståhl S, Uhlén M. Engineering proteins to facilitate bioprocessing. Trends Biotechnol. 1994 May;12(5):184-8. Review. PubMed PMID: 7764901.

Ståhl S, Nygren PÅ, Sjölander A, Uhlén M. Engineered bacterial receptors in immunology. Curr Opin Immunol. 1993 Apr;5(2):272-7. Review. PubMed PMID: 8507405.

Lorca T, Labbé JC, Devault A, Fesquet D, Strausfeld U, Nilsson J, Nygren PÅ, Uhlen M, Cavadore JC, Dorée M. Cyclin A-cdc2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs. J Cell Sci. 1992May;102 ( Pt 1):55-62. PubMed PMID: 1386852.

Murby M, Cedergren L, Nilsson J, Nygren PÅ, Hammarberg B, Nilsson B, Enfors SO, Uhlén M. Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy. Biotechnol ApplBiochem. 1991 Dec;14(3):336-46. PubMed PMID: 1777118.

Eliasson M, Andersson R, Nygren PÅ, Uhlén M. Structural and functional analysis of the human IgG-Fab receptor activity of streptococcal protein G. MolImmunol. 1991 Oct;28(10):1055-61. PubMed PMID: 1922101.

Murby M, Nygren PÅ, Rondahl H, Hellman U, Enfors SO, Uhlén M. Differential degradation of a recombinant albumin-binding receptor in Escherichia coli. Eur J Biochem. 1991 Jul 1;199(1):41-6. PubMed PMID: 1648486.

Sjölander A, Lövgren K, Ståhl S, Aslund L, Hansson M, Nygren PÅ, Larsson M, Hagstedt M, Wåhlin B, Berzins K, et al. High antibody responses in rabbits immunized with influenza virus ISCOMs containing a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA. Vaccine. 1991 Jun;9(6):443-50. PubMed PMID: 1887676.

Nygren PÅ, Ljungquist C, Trømborg H, Nustad K, Uhlén M. Species-dependent binding of serum albumins to the streptococcal receptor protein G. Eur J Biochem. 1990 Oct 5;193(1):143-8. PubMed PMID: 2171929.

Ståhl S, Sjölander A, Hansson M, Nygren PÅ, Uhlén M. A general strategy for polymerization, assembly and expression of epitope-carrying peptides applied to the Plasmodium falciparum antigen Pf155/RESA. Gene. 1990 May 14;89(2):187-93. PubMed PMID: 1695598.

Sjölander A, Ståhl S, Nygren PÅ, Aslund L, Ahlborg N, Wåhlin B, Scherf A, Berzins K, Uhlén M, Perlmann P. Immunogenicity and antigenicity in rabbits of a repeated sequence of Plasmodium falciparum antigen Pf155/RESA fused to two immunoglobulin G-binding domains of staphylococcal protein A. Infect Immun. 1990 Apr;58(4):854-9. PubMed PMID: 2180822; PubMed Central PMCID: PMC258551.

Ståhl S, Sjölander A, Nygren PÅ, Berzins K, Perlmann P, Uhlén M. A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA. J Immunol Methods. 1989 Nov 13;124(1):43-52. PubMed PMID: 2681427.

Hammarberg B, Nygren PÅ, Holmgren E, Elmblad A, Tally M, Hellman U, Moks T, Uhlén M. Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4367-71. PubMed PMID: 2543972; PubMed Central PMCID: PMC287270.

Nygren PÅ, Eliasson M, Abrahmsén L, Uhlén M, Palmcrantz E. Analysis and use of the serum albumin binding domains of streptococcal protein G. J Mol Recognit. 1988 Apr;1(2):69-74. PubMed PMID: 3273653.