Publikationer av Johan Rockberg
Refereegranskade
Artiklar
[1]
Mebrahtu, A., Aniander, G., Mega, A., Moradi, M., Thalén, N., Gudmundsdotter, L. ... Rockberg, J. (2024). Co-culture platform for tuning of cancer receptor density allows for evaluation of bispecific immune cell engagers. New Biotechnology, 79, 120-126.
[2]
Thalén, N., Karlander, M., Lundqvist, M., Persson, H., Hofström, C., Turunen, S. P. ... Rockberg, J. (2024). Mammalian cell display with automated oligo design and library assembly allows for rapid residue level conformational epitope mapping. Communications Biology, 7(1).
[3]
Thalén, N., Moradi, M., Lundqvist, M., Rodhe, J., Andersson, M., Bidkhori, G. ... Rockberg, J. (2024). Tuning of CHO secretional machinery improve activity of secreted therapeutic sulfatase 150-fold. Metabolic engineering, 81, 157-166.
[4]
Mega, A., Mebrahtu, A., Aniander, G., Ryer, E., Skold, A., Sandegren, A. ... Frejd, F. Y. (2023). A PDGFRB- and CD40-targeting bispecific AffiMab induces stroma-targeted immune cell activation. mAbs, 15(1).
[5]
Möller, M., Jönsson, M., Lundqvist, M., Hedin, B., Larsson, L., Larsson, E. ... Hober, S. (2023). An easy-to-use high-throughput selection system for the discovery of recombinant protein binders from alternative scaffold libraries. Protein Engineering Design & Selection, 36.
[6]
Moradi, M., Lundqvist, M., Harris, C., Malm, M., Volk, A.-L., Thalén, N. ... Rockberg, J. (2022). Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells. New Biotechnology, 68, 68-76.
[7]
Jönsson, M., Scheffel, J., Larsson, E., Möller, M., Rossi, G., Lundqvist, M. ... Hober, S. (2022). CaRA – A multi-purpose phage display library for selection of calcium-regulated affinity proteins. New Biotechnology, 72, 159-167.
[8]
Malm, M., Kuo, C.-C., Moradi, M., Mebrahtu, A., Wistbacka, N., Razavi, R. ... Rockberg, J. (2022). Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins. Metabolic engineering, 72, 171-187.
[9]
Saghaleyni, R., Malm, M., Moruzzi, N., Zrimec, J., Razavi, R., Wistbacka, N. ... Rockberg, J. (2022). Report Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells. Cell Reports, 39(11), 110936.
[10]
Hendrikse, N., Sandegren, A., Andersson, T., Blomqvist, J., Makower, A., Possner, D. ... Nordling, E. (2021). Ancestral lysosomal enzymes with increased activity harbor therapeutic potential for treatment of Hunter syndrome. ISCIENCE, 24(3).
[11]
Volk, A.-L., Mebrahtu, A., Ko, B. -., Lundqvist, M., Karlander, M., Lee, H. -. ... Rockberg, J. (2021). Bispecific Antibody Molecule Inhibits Tumor Cell Proliferation More Efficiently Than the Two-Molecule Combination. Drugs in R&D, 21(2), 157-168.
[12]
Ding, M., Malhotra, R., Ottosson, T., Lundqvist, M., Mebrahtu, A., Brengdahl, J. ... Holmberg Schiavone, L. (2021). Secretome screening reveals immunomodulating functions of IFNα-7, PAP and GDF-7 on regulatory T-cells. Scientific Reports, 16767.
[13]
von Witting, E., Lindbo, S., Lundqvist, M., Möller, M., Wisniewski, A., Kanje, S. ... Hober, S. (2021). Small Bispecific Affinity Proteins for Simultaneous Target Binding and Albumin-Associated Half-Life Extension. Molecular Pharmaceutics, 18(1), 328-337.
[14]
Malm, M., Saghaleyni, R., Lundqvist, M., Giudici, M., Chotteau, V., Field, R. ... Rockberg, J. (2020). Evolution from adherent to suspension : systems biology of HEK293 cell line development. Scientific Reports, 10(1).
[15]
Tegel, H., Dannemeyer, M., Kanje, S., Sivertsson, Å., Berling, A., Svensson, A.-S. ... Hober, S. (2020). High throughput generation of a resource of the human secretome in mammalian cells. New Biotechnology, 58, 45-54.
[16]
Zhan, C., Bidkhori, G., Schwarz, H., Malm, M., Mebrahtu, A., Field, R. ... Chotteau, V. (2020). Low Shear Stress Increases Recombinant Protein Production and High Shear Stress Increases Apoptosis in Human Cells. iScience, 23(11).
[17]
Nasi, A., McArdle, S., Gaudernack, G., Westman, G., Melief, C., Rockberg, J. ... Mangsbo, S. (2020). Reactive oxygen species as an initiator of toxic innate immune responses in retort to SARS-CoV-2 in an ageing population, consider N-acetylcysteine as early therapeutic intervention. Toxicology reports, 7, 768-771.
[18]
Schwarz, H., Zhang, Y., Zhan, C., Malm, M., Field, R., Turner, R. ... Chotteau, V. (2020). Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin. Journal of Biotechnology, 309, 44-52.
[19]
Eisenhut, P., Mebrahtu, A., Moradi Barzadd, M., Thalén, N., Klanert, G., Weinguny, M. ... Rockberg, J. (2020). Systematic use of synthetic 5'-UTR RNA structures to tune protein translation improves yield and quality of complex proteins in mammalian cell factories. Nucleic Acids Research, 48(20).
[20]
Lundqvist, M., Thalén, N., Volk, A.-L., Hansen, H. G., von Otter, E., Nygren, P.-Å. ... Rockberg, J. (2019). Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion. Scientific Reports, 9.
[21]
Hu, F. J., Lundqvist, M., Uhlén, M. & Rockberg, J. (2019). SAMURAI (Solid-phase Assisted Mutagenesis by Uracil Restriction for Accurate Integration) for antibody affinity maturation and paratope mapping. Nucleic Acids Research, 47(6).
[22]
Häussler, R. S., Bendes, A., Iglesias, M. J., Sanchez-Rivera, L., Dodig-Crnkovic, T., Byström, S. ... Schwenk, J. M. (2019). Systematic Development of Sandwich Immunoassays for the Plasma Secretome. Proteomics.
[23]
Uhlén, M., Karlsson, M. J., Hober, A., Svensson, A.-S., Scheffel, J., Kotol, D. ... Sivertsson, Å. (2019). The human secretome. Science Signaling, 12(609).
[24]
Nilvebrant, J. & Rockberg, J. (2018). An introduction to epitope mapping. Methods in Molecular Biology, 1785, 1-10.
[25]
Hu, F. J., Volk, A.-L., Persson, H., Säll, A., Borrebaeck, C., Uhlén, M. & Rockberg, J. (2018). Combination of phage and Gram-positive bacterial display of human antibody repertoires enables isolation of functional high affinity binders. New Biotechnology, 45, 80-88.
[26]
Zamani, L., Lundqvist, M., Zhang, Y., Aberg, M., Edfors, F., Bidkhori, G. ... Chotteau, V. (2018). High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome. Biotechnology Journal, 13(10).
[27]
Thul, P. J., Åkesson, L., Wiking, M., Mahdessian, D., Geladaki, A., Ait Blal, H. ... Lundberg, E. (2017). A subcellular map of the human proteome. Science, 356(6340).
[28]
Sastry, A., Monk, J., Tegel, H., Uhlén, M., Pålsson, B. O., Rockberg, J. & Brunk, E. (2017). Machine learning in computational biology to accelerate high-throughput protein expression. Bioinformatics, 33(16), 2487-2495.
[29]
Volk, A.-L., Hu, F. J., Berglund, M. M., Nordling, E., Strömberg, P., Uhlén, M. & Rockberg, J. (2016). Stratification of responders towards eculizumab using a structural epitope mapping strategy. Scientific Reports, 6.
[30]
Ko, B.-K., Lee, S.-Y., Lee, Y.-H., Hwang, I.-S., Persson, H., Rockberg, J. ... Lee, J.-S. (2015). Combination of novel HER2-targeting antibody 1E11 with trastuzumab shows synergistic antitumor activity in HER2-positive gastric cancer. Molecular Oncology, 9(2), 398-408.
[31]
Forsström, B., Axnäs, B. B., Rockberg, J., Danielsson, H., Bohlin, A. & Uhlén, M. (2015). Dissecting Antibodies with Regards to Linear and Conformational Epitopes. PLOS ONE, 10(3).
[32]
Anfelt, J., Kaczmarzyk, D., Shabestary, K., Renberg, B., Rockberg, J., Nielsen, J. ... Hudson, E. P. (2015). Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production. Microbial Cell Factories, 14.
[33]
Lundqvist, M., Edfors, F., Sivertsson, Å., Hallström, B. M., Hudson, E. P., Tegel, H. ... Rockberg, J. (2015). Solid-phase cloning for high-throughput assembly of single and multiple DNA parts. Nucleic Acids Research, 43(7).
[34]
Uhlén, M., Fagerberg, L., Hallström, B. M., Lindskog, C., Oksvold, P., Mardinoglu, A. ... Pontén, F. (2015). Tissue-based map of the human proteome. Science, 347(6220), 1260419.
[35]
Hansen, H. G., Nilsson, C. N., Lund, A. M., Kol, S., Grav, L. M., Lundqvist, M. ... Kildegaard, H. F. (2015). Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Scientific Reports, 5.
[36]
Hu, F. J., Uhlén, M. & Rockberg, J. (2014). Generation of HER2 monoclonal antibodies using epitopes of a rabbit polyclonal antibody. New Biotechnology, 31(1), 35-43.
[37]
Forsström, B., Axnäs, B. B., Stengele, K.-P., Buehler, J., Albert, T. J., Richmond, T. A. ... Uhlén, M. (2014). Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide Arrays. Molecular & Cellular Proteomics, 13(6), 1585-1597.
[38]
Hudson, E. P., Nikoshkov, A., Uhlén, M. & Rockberg, J. (2012). Automated Solid-Phase Subcloning Based on Beads Brought into Proximity by Magnetic Force. PLOS ONE, 7(5), e37429.
[39]
Buus, S., Rockberg, J., Forsström, B., Nilsson, P., Uhlén, M. & Schafer-Nielsen, C. (2012). High-resolution mapping of linear antibody epitopes using ultrahigh-density peptide microarrays. Molecular & Cellular Proteomics, 11(12), 1790-1800.
[40]
Hudson, E. P., Uhlén, M. & Rockberg, J. (2012). Multiplex epitope mapping using bacterial surface display reveals both linear and conformational epitopes. Scientific Reports, 2, 706.
[41]
Hjelm, B., Forsström, B., Löfblom, J., Rockberg, J. & Uhlén, M. (2012). Parallel Immunizations of Rabbits Using the Same Antigen Yield Antibodies with Similar, but Not Identical, Epitopes. PLOS ONE, 7(12), e45817.
[42]
Hjelm, B., Forsström, B., Igel, U., Johannesson, H., Stadler, C., Lundberg, E. ... Uhlen, M. (2011). Generation of monospecific antibodies based on affinity capture of polyclonal antibodies. Protein Science, 20(11), 1824-1835.
[43]
Rockberg, J., Löfblom, J., Hjelm, B., Ståhl, S. & Uhlén, M. (2010). Epitope mapping using gram-positive surface display. Current Protocols in Immunology(SUPPL. 90), 9.9.1-9.9.17.
[44]
Hjelm, B., Fernandez, C. D., Löfblom, J., Ståhl, S., Johannesson, H., Rockberg, J. & Uhlen, M. (2010). Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase. NEW BIOTECHNOL, 27(2), 129-137.
[45]
Kronqvist, N., Malm, M., Rockberg, J., Hjelm, B., Uhlén, M., Ståhl, S. & Löfblom, J. (2010). Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies. Recent Patents on Biotechnology, 4(3), 171-182.
[46]
Rockberg, J., Schwenk, J. M. & Uhlén, M. (2009). Discovery of epitopes for targeting the human epidermal growth factor receptor 2 (HER2) with antibodies. Molecular Oncology, 3(3), 238-247.
[47]
Kronqvist, N., Rockberg, J., Hjelm, B., Ståhl, S., Uhlén, M. & Löfblom, J. (2009). New Ways for Discovery of Biopharmaceuticals : Emerging Techniques using Surface Display on Gram-positive Bacteria for Combinatorial Protein Engineering and Characterization. Bioforum Europe, 13(6-7), 022.
[48]
Rockberg, J. & Uhlén, M. (2009). Prediction of antibody response using recombinant human protein fragments as antigen. Protein Science, 18(11), 2346-2355.
[49]
Berglund, L., Björling, E., Jonasson, K., Rockberg, J., Fagerberg, L., Al-Khalili Szigyarto, C. ... Uhlén, M. (2008). A whole-genome bioinformatics approach to selection of antigens for systematic antibody generation. Proteomics, 8(14), 2832-2839.
[50]
Rockberg, J., Löfblom, J., Hjelm, B., Ståhl, S. & Uhlén, M. (2008). Epitope mapping of antibodies using bacterial surface display. Nature Methods, 5(12), 1039-1045.
[51]
Uhlén, M., Björling, E., Agaton, C., Al-Khalili Szigyarto, C., Amini, B., Andersen, E. ... Pontén, F. (2005). A human protein atlas for normal and cancer tissues based on antibody proteomics. Molecular & Cellular Proteomics, 4(12), 1920-1932.
[52]
Lindskog, M., Rockberg, J., Uhlén, M. & Sterky, F. (2005). Selection of protein epitopes for antibody production. BioTechniques, 38(5), 723-727.
Kapitel i böcker
[53]
Volk, A.-L. & Rockberg, J. (2018). Discontinuous epitope mapping of antibodies using bacterial cell surface display of folded domains. I Epitope Mapping Protocols (s. 159-183). Humana Press.
[54]
Volk, A.-L., Hu, F. J. & Rockberg, J. (2018). Epitope mapping of antibodies using bacterial cell surface display of gene fragment libraries. I Epitope Mapping Protocols (s. 141-157). Humana Press.
[55]
Volk, A. L., Hu, F. J. & Rockberg, J. (2014). Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display. I Monoclonal antibodies (s. 485-500). Humana Press.
Icke refereegranskade
Artiklar
[56]
Malm, M., Volk, A.-L., Karlander, M., Lundqvist, M., Wistbacka, N., Razavi, R. ... Rockberg, J. (2021). Improving targeting and yield of AAV by capsid and cell engineering. Human Gene Therapy, 32(19-20), A119-A120.
[57]
Rockberg, J. (2016). Split-GFP and droplet microfluidics allows high-throughput screening of mammalian cell factories. New Biotechnology, 33, S51-S51.
[58]
Rockberg, J., Szigyarto, C. A. & Uhlén, M. (2004). Antigen selection for design of protein epitope signature tags. Molecular & Cellular Proteomics, 3(10), S5-S5.
[59]
Szigyarto, C. A., Berglund, L., Sivertsson, Å., Lindskog, M., Rockberg, J., Westberg, J. ... Uhlén, M. (2004). Statistics of protein epitope signature tag design within the Swedish human proteom resource. Molecular & Cellular Proteomics, 3(10), S91-S91.
[60]
Sterky, F., Berglund, L., Lindskog, M., Rockberg, J., Al-Khalili Szigyarto, C. & Uhlén, M. (2004). Strategies and software for design of protein Epitopes within HPR. Molecular & Cellular Proteomics, 3(10), S4-S4.
Konferensbidrag
[61]
Rockberg, J., Nilvebrant, J. (2018). Preface. I Methods in Molecular Biology: -. (s. v-vi). Humana Press.
Avhandlingar
[62]
Rockberg, J. (2008). Methods for Generation and Characterization of Monospecific Antibodies (Doktorsavhandling , KTH, Stockholm, Trita-BIO-Report 2008:20). Hämtad från http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-9338.
Övriga
[63]
Mega, A., Mebrahtu, A., Aniander, G., Ryer, E., Sköld, A., Sandegren, A., Backström Rydin, E., Rockberg, J., Östman, A., Y. Frejd, F. (). A PDGFRB and CD40 targeting bispecific AffiMab induces stroma targeted immune cell activation. (Manuskript).
[64]
Thorell, H., Karlander, M., Thalén, N., Ryner, M., Fresk, M., Villacañas González, M. D. C., Jung, T., Douthwaite, J., Cusdin, F., Hatton, D., Malm, M., Rockberg, J. (). A modular binding domain AAV platform for cell surface receptor-selective DNA delivery. (Manuskript).
[65]
von Witting, E., Lindbo, S., Lundqvist, M., Möller, M., Wisniewski, A., Kanje, S., Rockberg, J., Tegel, H., Åstrand, M., Uhlén, M., Hober, S. (). ADAPT as a single-domain bispecific scaffold capable of albumin-associated haf-life extension for therapeutic applications. (Manuskript).
[66]
Karlander, M., Karlsson, A., Ryner, M., Jung, T., Bausewein, T., Coulomb-Delsuc, M., Wistbacka, N., Grassi, L., Cusdin, F., Douthwaite, J., Hatton, D., Malm, M., Rockberg, J. (). Amplification and Fragmentation Free Long-read Sequencing Enables Rapid Analysis of Packaged Adeno-associated Virus ssDNA. (Manuskript).
[67]
Mebrahtu, A., Lauren, I., Veerman, R. E., Güclüler Akpinar, G., Lord, M., Kostakis, A., Astorga-Wells, J., Dahllund, L., Olsson, A., Persson, J., Persson, H., Pierre, D., Rockberg, J., Mangsbo, S. (). An Adaptable Drug Affinity Conjugate targeting CD40 for modular non-covalent delivery of neoantigens providing robust T-cell activation and increased overall survival in tumor-bearing mice. (Manuskript).
[68]
Moradi, M., Lundqvist, M., Harris, C., Malm, M., Volk, A.-L., Thalén, N., Chotteau, V., Grassi, L., Smith, A., Abadi, M. L., Lambiase, G., Gibson, S., Hatton, D., Rockberg, J. (). Autophagy and intracellular product degradation genes reduce aggregation of bispecific antibody in CHO cells with a high translational burden. (Manuskript).
[69]
Jonsson, M., Scheffel, J., Larsson, E., Möller, M., Rossi, G., Lundqvist, M., Rockberg, J., Uhlén, M., Tegel, H., Kanje, S., Hober, S. (). CaRA – A Multi-Purpose Phage Display Library for Selection of Calcium-Regulated Affinity Proteins. (Manuskript).
[70]
Mebrahtu, A., Svensson Gelius, S., Su, C., M. Berglund, M., Strömberg, P., Cornevik, T., Elorant, M.-L., Rönnblom, L., Parodis, I., Brodin, P., Y. Frejd, F., Rockberg, J. (). Disrupting the hyperactive inflammatory cycle in SLE - A bispecific drug targeting C5 and IFNa in the Complement and Innate immune system. (Manuskript).
[71]
Aniander, G., Mirazadeh, K., Thalén, N., Volk, A.-L., Samuelson, P., Rockberg, J. (). Enhanced titer and quality of IgGs through alterations at the translation initiation sequence. (Manuskript).
[72]
Malm, M., Kuo, C.-C., Moradi Barzadd, M., Mebrahtu, A., Wistbacka, N., Razavi, R., Volk, A.-L., Lundqvist, M., Kotol, D., Edfors, F., Gräslund, T., Chotteau, V., Field, R., Varley, P. G., Roth, R. G., Lewis, N. E., Hatton, D., Rockberg, J. (). Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins. (Manuskript).
[73]
Zhan, C., Hubert, S., Malm, M., Sellick, C., Rockberg, J., Chotteau, V. (). Hydrodynamic shear stress in hollow filter for perfusion culture of human cells. (Manuskript).
[74]
Thalén, N., Lundqvist, M., Persson, H., Hofström, C., Turunen, P., Godzwon, M., Volk, A.-L., Ohlin, M., Rockberg, J. (). Mammalian cell surface display system for conformational epitope determination of SARS-CoV-2 neutralizing antibodies. (Manuskript).
[75]
Wisniewski, A., Larsson, L., Lundqvist, M., Rockberg, J., Tegel, H., Uhlén, M., Hober, S. (). Single domain ADAPT able to target Interleukin-17c with high affinity and albumin simultaneously for potential half-life extension. (Manuskript).
[76]
Schwarz, H., Zhang, Y., Zhan, C., Malm, M., Field, R., Turner, R., Sellick, C., Varley, P., Rockberg, J., Chotteau, V. (). Small-scale bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin. (Manuskript).
[77]
Karlander, M., Rönning, S., Lundqvist, M., Malm, M., Rockberg, J. (). Survival of the less fit - directed evolution via deep sequencing enables selection of diverse high affinity proteins. (Manuskript).
[78]
Thalén, N., Moradi, M., Lundqvist, M., Rodhe, J., Andersson, M., Bidkori, G., Possner, D., Su, C., Nilsson, J., Eisenhut, P., Malm, M., Westin, J., Forsberg, J., Nordling, E., Mardinoglu, A., Volk, A.-L., Sandegren, A., Rockberg, J. (). Systems biology greatly improve activity of secreted therapeutic sulfatase in CHO bioprocess. (Manuskript).
[79]
Mebrahtu, A., Aniander, G., Mega, A., Moradi, M., Thalén, N., Gudmundsdotter, L., Backström Rydin, E., Sandegren, A., Frejd, F. Y., Rockberg, J. (). Tuning of PDGFRB density on cell surface allows for selective B cell activation with CD40-targeting bi-specific antibody. (Manuskript).
[80]
Thalén, N., Hicks, J., Lundqvist, M., Backmark, A., Engberg, S., Raynoschek, C., Gunnarsson, J., Volk, A.-L., Roth, R., Rockberg, J. (). ULK1 knockout cell line downregulates autophagy, upregulates recombinant transcript and improves protein secretion. (Manuskript).
Senaste synkning med DiVA:
2024-11-18 00:03:12