Publications
ORCID: orcid.org/0000-0002-5805-2693
ResearcherID: E-8646-2010
Publications
53. Xu X, Williams TC, Divne C, Pretorius IS, Paulsen IT (2019) Evolutionary engineering in Saccharomyces cerevisiae reveals a TRK1-dependent potassium influx mechanism for propionic acid tolerance. Biotechnol Biofuels 12:97.
52. Quehenberger J, Reichenbach T, Baumann N, Rettenbacher L, Divne C, Spadiut O (2019) Kinetics and predicted structure of a novel xylose reductase from Chaetomium thermophilum. Int. J. Mol. Sci. 20, 1-17.
51. Reichenbach T, Kalyani D, Gandini R, Svartström O, Aspeborg H, Divne C (2018)
Structural and biochemical characterization of the Cutibacterium acnes exo-β-1,4-mannosidase that targets the N-glycan core of host glycoproteins
PLoS One 13:e0204703.
KTH Press release in Swedish
Ciennce Press release in Swedish
KTH Press release in English
50. Gandini R, Reichenbach T, Tan TC & Divne C (2017) Structural basis for dolichylphosphate mannose biosynthesis. Nature Communications 8,120.
KTH press release in Swedish
KTH press release in English
49. Hassan, N., Geiger, B., Gandini, R., Patel, B.K.C., Kittl, R., Haltrich, D., Nguyen, T.H., Divne, C. & Tan T.C. (2016) Engineering a thermostable Halothermothrix orenii beta-glucosidase for improved galacto-oligosaccharide synthesis. Appl. Microbiol. Biotechnol. 100, 3533–3543.
48. Tan, T.C., Kracher, D., Gandini, R., Sygmund, C., Kittl, R., Haltrich, D., Hällberg, B.M., Ludwig, R. & Divne, C. (2015). Structural basis for cellobiose dehydrogenase action during oxidative cellulose degradation. Nature Communications 6, 7542.
47. Wang, Y., Azhar, S., Gandini, R., Divne, C., Ezcurra, I. & Aspeborg, H. (2015). Biochemical characterization of the novel endo-beta-mannanase AtMan5-2 from Arabidopsis thaliana. Plant Science 241, 151-163.
46. Hassan, N., Kori, L.D., Gandini, R., Patel, B.K., Divne, C. & Tan, T.C. (2015). High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with alpha-L-arabinofuranosidase activity. Acta Cryst. Sect. F71, 338–345.
45. Hassan, N., Nguyen, T.H., Intanon, M., Kori, L.D., Patel, B.K., Haltrich, D., Divne, C. & Tan, T.C. (2015). Biochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis. Appl. Microbiol. Biotechnol. 99, 1731–1744.
44. Ullmann, E., Tan, T.C., Gundinger, T., Herwig, C., Divne, C. & Spadiut, O. (2014). A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis. Biosci. Rep. 34, 893–904.
43. Tan, T.C., Spadiut, O., Gandini, R., Haltrich, D. & Divne, C. (2014). Structural basis for binding of fluorinated glucose and galactose to Trametes multicolor pyranose 2-oxidase variants with improved galactose conversion. PLOS ONE, 9, e86736.
42. Hassan, N., Tan, T.C., Spadiut, O., Peterbauer, C., Haltrich, D. & Divne, C. (2013). Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists homotetramer assembly. FEBS Open Bio, 3, 496-504.
41. Tan, T.C., Spadiut, O., Wongnate, T., Sucharitakul, J., Krondorfer, I., Sygmund, C., Haltrich, D., Chaiyen, P., Peterbauer, C.K. & Divne, C. (2013). The 1.6 ångstrom crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate. PLOS ONE, 8, e53567.
40. Tan, T.C., Haltrich, D. & Divne, C. (2011). Regioselective control of b-D-glucose oxidation by pyranose 2-oxidase is intimately coupled to conformational degeneracy. J. Mol. Biol. 409, 588-600.
39. Tan, T.C., Pitsawong, W., Spadiut, O., Haltrich, D., Chaiyen, P. & Divne, C. (2010) H-bonding and positive charge at the N(5)/O(4) locus are critical for covalent flavin attachment in Trametes pyranose 2-oxidase. J. Mol. Biol. 402, 578-594.
38. Spadiut, O., Tan, T.C., Pisanelli, I., Haltrich, D. & Divne, C. & (2010). Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase. FEBS J, 277, 2892-2909.
37. Pitsawong, W., Sucharitakul, J. Prongjit, M., Tan, T.C., Spadiut, O., Haltrich, D., Divne, C. & Chaiyen, P. (2010). A conserved active-site threonine is important for both half-reactions of pyranose 2-oxidase. J. Biol. Chem. 285, 9697-9705.
36. Gullfot, F., Tan, T.C., von Schantz, L., Nordberg-Karlsson, E., Ohlin, M., Brumer, H. & Divne, C. (2010). The crystal structure XG-34, an evolved xyloglucan-specific carbohydrate-binding module. Proteins, 78, 785-789.
35. Eklöf, J., Tan, T.C., Divne, C. & Brumer, H. (2009). The crystal structure of the outer membrane lipoprotein YbhC from Escherichia coli sheds new light on the phylogeny of Carbohydrate Esterase Family8. Proteins, 76, 1029-1036.
34. Salaheddin, C., Spadiut, O., Ludwig, R., Tan, T.C., Divne, C., Haltrich, D. & Peterbauer, C. (2009). Probing active-site residues of pyranose 2-oxidase from Trametes multicolor by semi-rational protein design. Biotechnol. J. 4, 535-543.
33. Spadiut, O., Radakovits, K., Pisanelli, I., Salaheddin, C., Yamabhai, M., Tan, T.C., Divne, C. & Haltrich, D. (2009). A thermostable triple mutant of pyranose 2-oxidase from Trametes multicolor with improved properties for biotechnological applications. Biotechnol. J. 4, 525-534.
32. Spadiut, O., Leitner, C., Salaheddin, C., Varga, B., Vertessy, B., Tan, T.C., Divne, C. & Haltrich, D. (2009). Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design. FEBS J., 276, 776-792.
31. Rajangam, A.S., 15 authors (2008). MAP20, a microtubule-associated protein in the secondary cell wall of Populus tremula L. x tremuloides Michx is a target of the cellulose biosynthesis inhibitor, 2,6-dichlorobenzonitrile. Plant Physiol. 148, 1283-1294.
30. Tan, T.C, Mijts, B.N., Swaminathan, K., Patel, B.K.C., & Divne, C. (2008). Crystal structure of the polyextremophilic alpha-amylase AmyB from Halothermothrix orenii: details of a productive enzyme-substrate complex and an N domain with a role in binding raw starch. J. Mol. Biol. 378, 850-868.
29. Kittl, R., Sygmund, C., Halada, P., Volc, J., Divne, C., Haltrich, D., & Peterbauer, C.K. (2008). Molecular cloning of three pyranose dehydrogenase-encoding genes from Agaricus meleagris and analysis of their expression by real-time RT-PCR. Curr. Genet. 53, 117-127.
28. Spadiut, O., Leitner, C., Tan, T.C., Ludwig, R., Divne, C., & Haltrich, D. (2008). Mutations of Thr169 affect substrate specificity of pyranose 2-oxidase from Trametes multicolor. Biocat. Biotran. 26, 120-127.
27. Kujawa, M., Volc, J., Halada, P., Sedmera, P., Divne, C., Leitner, C., Peterbauer, C., & Haltrich, D. (2007). Properties of pyranose dehydrogenase purified from the litter-degarding fungus Agaricus xanthoderma. FEBS J. 274, 879-894.
26. Nguyen, T-H, Splechtna, B., Krasteva, S., Kneifel, W., Kulbe, K. D., Divne, C. & Haltrich, D. (2007). Characterization and molecular cloning of a heterodimeric β-galactosidase from the probiotic strain Lactobacillus acidophilus R22. FEMS Microbiol. Lett. 269, 136-144.
25. Kujawa, M., 12 authors & Divne C. (2006). Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. J. Biol. Chem. 281, 35104-35115.
24. Zamocky, M., Ludwig, R., Peterbauer, C., Hallberg, B.M., Divne, C., Nicholls, P. & Haltrich, D. (2006). Cellobiose dehydrogenase - a flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi. Curr. Protein Pept. Sci., 7, 255-280.
23. Master E.R., Rudsander U.J., Zhou W., Henriksson H., Divne C., Denman S., Wilson D.B. & Teeri T.T. (2004). Recombinant expression and enzymatic characterization of PttCel9A, a KOR homolog from Populus tremula X Populus tremuloides. Biochemistry, 43, 10080-10089.
22. Hallberg B.M., Leitner C., Haltrich D. & Divne C. (2004). Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase. J. Mol. Biol. 341, 781-796.
21. Zamocky, M., Hallberg, B.M., Ludwig, R., Divne, C. & Haltrich, D. (2004). Ancestral gene fusion in cellobiose dehydrogenase reflects a specific evolution of GMC oxidoreductases in fungi. Gene, 338, 1-14.
20. Hallberg, B.M., Leitner, C., Haltrich, D. & Divne, C. (2004). Crystallization and preliminary X-ray diffraction analysis of pyranose 2-oxidase from the white-rot fungus Trametes multicolor. Acta Crystallogr. sect. D Biol Crystallogr. 60, 197-199.
19. von Ossowski, I., 14 authors (2003). Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. J. Mol. Biol. 333, 817-829.
18. Rotsaert, F.A., Hallberg, B.M., De Vries, S., Moenne-Loccoz, P., Divne, C., Renganathan, V., Gold, M.H. (2003). Biophysical and structural analysis of a novel heme b iron ligation in the flavocytochrome cellobiose dehydrogenase. J. Biol. Chem. 278, 33224-33231.
17. Mason, M.G., Nicholls, P., Divne, C., Hallberg, B.M., Henriksson, G. & Wilson, M.T. (2003). The heme domain of cellobiose oxidoreductase: a one-electron reducing system. Biochim. Biophys.Acta 1604, 47-54.
16. Hallberg, B.M., Henriksson, G., Pettersson, G., Vasella, A. & Divne, C. (2003). Mechanism of the reductive half reaction in cellobiose dehydrogenase. J. Biol. Chem. 278, 7160-7166.
15. Hallberg, B.M., Henriksson, G., Pettersson, G. & Divne, C. (2002). Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase. J. Mol. Biol. 315, 421-434.
14. Yoshida, M., Ohira, T., Igarashi, K., Nagasawa, H., Aida, K., Hallberg, B.M., Divne, C., Nishino, T. & Samejima, M. (2001). Production and characterization of recombinant Phanerochaete chrysosporium cellobiose dehydrogenase in the methylotrophic yeast Pichia pastoris. Biosci. Biotechnol. Biochem. 65, 2050-2057.
13. Becker, D., 17 authors (2001). Engineering of a glycosidase family 7 cellobiohydrolase to more alkaline pH optimum: the behaviour of Trichoderma reesei CBHI and its E223S, A224H, L225V, T226A, D262G mutants. Biochemical J. 356, 19-30.
12. Ståhlberg, J., Henriksson, H., Divne, C., Isaksson, R., Pettersson, G., Johansson, G. & Jones, T.A. (2001). Structural basis for enantiomer binding and separation of a common beta-blocker: crystal structure of cellobiohydrolase Cel7A with bound (S)-propranolol at 1.9 Å resolution. J. Mol. Biol. 305, 79-93.
11. Hallberg, B.M., Bergfors, T., Bäckbro, K., Pettersson, G., Henriksson, G. & Divne, C. (2000). A new scaffold for binding of haem in the cytochrome domain of the extracellular flavocytochrome cellobiose dehydrogenase. Structure, 8, 79-88.
10. MacKenzie, L.F., Sulzenbacher, G., Divne, C., Jones, T.A., Woldike, H.F., Schulein, M., Withers, S.G. & Davies, G.J. (1998). Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 Å resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate. Biochem. J. 335, 409-416.
9. Teeri, T.T., Koivula, A., Linder, M., Wohlfahrt, G., Divne, C. & Jones, T.A. (1998). Trichoderma reesei cellulases: why so efficient on crystalline cellulose? Biochem. Soc. Trans. 26, 173-178.
8. Divne, C., Ståhlberg, J., Teeri, T.T. & Jones, T.A. (1998). High-resolution crystal structures reveal how a cellulose chain is bound in the 50Å long tunnel of cellobiohydrolase I from Trichoderma reesei. J. Mol. Biol. 275, 309-325.
7. Henriksson, G., Salumets, A., Divne, C. & Petterson, G. (1997). Studies of cellulose binding by cellobiose dehydrogenase and a comparison with cellobiohydrolase I. Biochem J. 324, 833-838.
6. Henriksson, H., Ståhlberg, J., Koivula, A., Pettersson, G., Divne, C., Valtcheva, L. & Isaksson, R. (1997). The catalytic amino-acid residues in the active site of cellobiohydrolase I are involved in chiral recognition. J. Biotechnol. 57, 115-125.
5. Kleywegt, G.J., Zou, J.-Y., Divne, C., Davies, G.J., Sinning, I., Ståhlberg, J., Reinikainen, T., Srisodsuk, M., Teeri, T.T. & Jones, T.A. (1997). The crystal structure of the catalytic domain of endoglucanase I from Trichoderma reesei at 3.6 Å resolution, and a comparison with related enzymes. J. Mol. Biol. 272, 383-397.
4. Ståhlberg, J., Divne, C., Koivula, A., Piens, K., Claeyssens, M., Teeri, T.T. & Jones, T.A. (1996). Activity studies and crystal structures of catalytically deficient mutants of Cellobiohydrolase I. J. Mol. Biol. 264, 337-349.
3. Raices, M., Paifer, E., Cremata, J., Montesino, R., Ståhlberg, J., Divne, C., Szabó, I.J., Henriksson, G., Johansson, G. & Pettersson, G. (1995). Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium. FEBS Lett. 369, 233-238.
2. Divne, C., Ståhlberg, J., Reinikainen, T., Ruohonen, L., Pettersson, G., Knowles, J.K.C., Teeri, T.T. & Jones, A. (1994). The three-dimensional structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. Science, 265, 524-528.
1. Divne, C., Sinning, I., Ståhlberg, J., Pettersson, G., Bailey, M., Siika-aho, M., Margolles-Clark, E., Teeri, T. & Jones, T.A. (1993). Crystallization and preliminary X-ray studies on the core proteins of cellobiohydrolase I and endoglucanase I from Trichoderma reesei. J. Mol. Biol. 234, 905-907.
PhD thesis 1994
Divne, C. (1994). “The three-dimensional structure of cellobiohydrolase I from Trichoderma reesei. Ph.D Thesis, Uppsala University, Repro HSC, Uppsala, Sweden.”
